Part:BBa_K3956014:Design

When building the plasmid to express Scl2, we had to add a 5’UTR in front of the codon to make sure the triple helical structure was produced, as well as making sure any signaling peptides were removed, as scl2 is a surface adhesion protein in S. pyogenes.

References: Peng, Y.Y., Howell, L., Stoichevska, V. et al. Towards scalable production of a collagen-like protein from Streptococcus pyogenes for biomedical applications. Microb. Cell Fact 11, 146 (2012). https://doi.org/10.1186/1475-2859-11-146

Curran, K. A., Karim, A. S., Gupta, A., & Alper, H. S. (2013). Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications. Metabolic engineering, 19, 88–97. https://doi.org/10.1016/j.ymben.2013.07.001

Squeglia, F., Bachert, B., Lukomski S., et al. (2014). The Crystal Structure of the Streptococcal Collagen-like Protein 2 Globular Domain from Invasive M3-type Group A Streptococcus Shows Significant Similarity to Immunomodulatory HIV Protein gp41*. Journal of Biological Chemistry, 289, 8. https://doi.org/10.1074/jbc.M113.523597